Adam M. Feist

Research Scientist

July 2010 – present

Grants and Awards

  • Scientific Lead, Host Engineering - JBEI Agency: DOE
  • Co-I - Agency: NIH - Focus: Systems biology targeting of antimicrobial resistance Project Description
  • PI - Agency: Novo Nordisk Foundation - Focus: Automated Adaptive Laboratory Evolution (ALE) technology development
  • Co-PI - Agency: Novo Nordisk Foundation - Focus: Network Reconstruction and in silico Biology
  • Co-I - Agency: NIH - Focus: Systems biology targeting of antimicrobial resistance Project Description


Key Selected Publications

  • Sandberg TE, Salazar MJ, Weng LL, Palsson BO, Feist AM “The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.” Metab Eng. 2019 Aug 8. pii: S1096-7176(19)30153-3. doi: 10.1016/j.ymben.2019.08.004

  • Guzmán GI, Sandberg TE, LaCroix RA, Nyerges A, Papp H, de Raad M, King ZA, Hefner Y, Northen TR, Notebaart RA, Pál C, Palsson BO, Papp B, Feist AM “Enzyme promiscuity shapes adaptation to novel growth substrates” Mol Syst Biol. 2019 15: e8462 doi: 10.15252/msb.20188462

  • Guzman GI, Olson CA, Hefner Y, Phaneuf PV, Catoiu E, Crepaldi LB, Goldschmidt L, Palsson BO, Feist AM “Reframing gene essentiality in terms of adaptive flexibility” BMC Syst Biol. Dec 17;12(1):143. doi: 10.1186/s12918-018-0653-z. (2018)

  • Phaneuf PV, Gosting D, Palsson BO, Feist AM. “ALEdb 1.0: a database of mutations from adaptive laboratory evolution experimentation.” Nucleic Acids Res. Oct 24. doi: 10.1093/nar/gky983. (2018)

  • McCloskey D, Xu S, Sandberg TE, Brunk E, Hefner Y, Szubin R, Feist AM, Palsson BO. “Adaptive laboratory evolution resolves energy depletion to maintain high aromatic metabolite phenotypes in Escherichia coli strains lacking the Phosphotransferase System.” Metab Eng. June 15. doi: 10.1016/j.ymben.2018.06.005 (2018)

  • Sandberg TE, Lloyd CJ, Palsson BO, Feist AM, “Laboratory Evolution to Alternating Substrate Environments Yields Distinct Phenotypic and Genetic Adaptive Strategies.” Appl Environ Microbiol. Apr 28. pii: AEM.00410-17. doi: 10.1128/AEM.00410-17. (2017)

  • LaCroix RA, Palsson BO, Feist AM “A Model for Designing Adaptive Laboratory Evolution Experiments.” Appl Environ Microbiol. Mar 31;83(8). pii: e03115-16. doi: 10.1128/AEM.03115-16 (2017)

  • Guzman GI, Utrilla J, Brunk E, Monk JM, Ebrahim A, Nurk S, Palsson BO, Feist A.M. "Model-driven discovery of underground metabolic functions in Escherichia coli." Proc Natl Acad Sci U S A. 112(3):929-34. (2015)

  • LaCroix RA, Sandberg TE, O'Brien EJ, Utrilla J, Ebrahim A, Guzman GI, Szubin R, Palsson BO, Feist A.M. “Discovery of key mutations enabling rapid growth of Escherichia coli K-12 MG1655 on glucose minimal media using adaptive laboratory evolution.” Applied and Environmental Biology, 18(1):17-30 (2015)

  • Campodonico MA, Andrews BA, Asenjo JA, Palsson BO, Feist A.M. “Generation of an atlas for commodity chemical production in Escherichia coli and a novel pathway prediction algorithm, GEM-Path.” Metabolic Engineering 25:140-58 (2014)

Google Scholar

Research Focuses and Skills:

  • Adaptive Laboratory Evolution (ALE) - Supervises and leads the design, development, and implementation of an automated platform for ALE studies and a bioinformatics-driven mutational database
  • Constraint-based Modeling and Reconstruction (COBRA): Major contributor to the field through model building, algorithm development, applied studies, and experimental validation
  • Runs day-to-day operations of the Systems Biology Research Group including managing budgets and wet lab
  • Serves as Research Advisor and Thesis Committee Member for numerous graduate students in the department of Bioengineering, as well as mentoring Post-docs and Undergraduate students


Doctor of Philosophy in Bioengineering University of California - San Diego, December 2008

Master of Science in Bioengineering University of California - San Diego, December 2005

Bachelor of Science in Chemical Engineering University of Nebraska - Lincoln, May 2003, Honors Program Graduate

Previous Research Experience

GT Life Sciences, Inc., San Diego, CA (2009 - 2010)

Biological Process Development Facility / UNL - Protein Purification Laboratory (2002 - 2003)

Goodyear Inc., Lincoln, Nebraska - Co-Op Participant (2001 - 2002)

Research Experience for Undergraduates Program (REU) / Colorado State University (Summer 2001)

Featured Media

Contact Information

Email Address:

Phone: (858) 534-9592

Mailing Address: 9500 Gilman Drive, Mail Code 0412, La Jolla, CA 92093-0412