Genome sequence of "BOP27", Wild-type E. coli K-12 MG1655 for SBRG

The community standard E. coli K-12 MG1655 'wild-type' sequence can be found here, although there is probably no actual strain with this genome. BOP27 is a wild-type Escherichia coli strain used in many studies conducted in the Palsson lab. The sequence of BOP27 is highly similar to the community standard E. coli K-12 MG1655 wild-type sequence. Recently, we sequenced BOP27 and used breseq to characterize its mutations compared to the reference sequence. Here we place the results in the context of a manuscript looking at Genetic Variations in Common Escherichia coli MG1655 Stock Cultures.

  • The findings are summarized below in an image:

 

Here, Table 1 derives from this manuscript.

The inversion mentioned above is interesting as we see both the reference sequence and the inverted sequence in most sequencing runs, even those sequenced from a single colony. Below is another image with some details about the original (O1,O2) and new junctions (J1,J2) we find:

 

Please contact Ali Ebrahim or Joshua Lerman for more information.

 

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