Scalable method to determine mutations that occur during adaptive evolution of Escherichia coli.

Title Scalable method to determine mutations that occur during adaptive evolution of Escherichia coli.
Year of Publication 2003
Authors A. Raghunathan; B.Ø. Palsson
Journal PLoS Comput Biol
Abstract Denaturing HPLC was used to determine mutations occurring during the adaptive evolution of Escherichia coli K-12. The strains were evolved over 700 generations on glycerol as the sole carbon source from a sub-optimal to an optimal growth rate. The mutations detected by direct sequencing of amplicons of the glycerol-phosphate regulon repressor (glpR) gene were a synonymous substitution Val20Val in two separately evolved strains. Non-synonymous substitutions, Val119Gly and Gly179Trp, were also observed in each of the two strains. This procedure can be scaled to determine genome-scale sequence variations that have occurred during adaptive evolution.
URL PubMed