Next-generation genome-scale models for metabolic engineering.

TitleNext-generation genome-scale models for metabolic engineering.
Publication TypeJournal Article
Year of Publication2015
AuthorsKing ZA, Lloyd CJ, Feist AM, Palsson BO
JournalCurr Opin Biotechnol
Volume35C
Pagination23-29
PubMed Date06/2015
ISSN1879-0429
Abstract

Constraint-based reconstruction and analysis (COBRA) methods have become widely used tools for metabolic engineering in both academic and industrial laboratories. By employing a genome-scale in silico representation of the metabolic network of a host organism, COBRA methods can be used to predict optimal genetic modifications that improve the rate and yield of chemical production. A new generation of COBRA models and methods is now being developed-encompassing many biological processes and simulation strategies-and next-generation models enable new types of predictions. Here, three key examples of applying COBRA methods to strain optimization are presented and discussed. Then, an outlook is provided on the next generation of COBRA models and the new types of predictions they will enable for systems metabolic engineering.

Alternate JournalCurr. Opin. Biotechnol.
PubMed ID25575024
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