Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli.

TitleDeciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli.
Publication TypeJournal Article
Year of Publication2014
AuthorsSeo SWoo, Kim D, Latif H, O'Brien EJ, Szubin R, Palsson BO
JournalNat Commun
Volume5
Pagination4910
PubMed Date09/2014
ISSN2041-1723
Abstract

The ferric uptake regulator (Fur) plays a critical role in the transcriptional regulation of iron metabolism. However, the full regulatory potential of Fur remains undefined. Here we comprehensively reconstruct the Fur transcriptional regulatory network in Escherichia coli K-12 MG1655 in response to iron availability using genome-wide measurements. Integrative data analysis reveals that a total of 81 genes in 42 transcription units are directly regulated by three different modes of Fur regulation, including apo- and holo-Fur activation and holo-Fur repression. We show that Fur connects iron transport and utilization enzymes with negative-feedback loop pairs for iron homeostasis. In addition, direct involvement of Fur in the regulation of DNA synthesis, energy metabolism and biofilm development is found. These results show how Fur exhibits a comprehensive regulatory role affecting many fundamental cellular processes linked to iron metabolism in order to coordinate the overall response of E. coli to iron availability.

Alternate JournalNat Commun
PubMed ID25222563
Cover Image: 

Location

Location

417 Powell-Focht Bioengineering Hall

9500 Gilman Drive La Jolla, CA 92093-0412

Contact Us

Contact Us

In Silico Lab:  858-822-1144

Wet Lab:  858-246-1625

FAX:   858-822-3120

Website Concerns: sbrgit@ucsd.edu

User Login