|Title||Metabolic network analysis integrated with transcript verification for sequenced genomes.|
|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Manichaikul A, Ghamsari L, Hom EFY, Lin C, Murray RR, Chang RL, Balaji S, Hao T, Shen Y, Chavali AK, Thiele I, Yang X, Fan C, Mello E, Hill DE, Vidal M, Salehi-Ashtiani K, Papin JA|
|PubMed Date||2009 Aug|
|Keywords||Animals, Chlamydomonas reinhardtii, Computational Biology, Computer Simulation, Enzymes, Genetic Engineering, Genome, Protozoan, Models, Genetic, Protozoan Proteins, Transcription, Genetic|
With sequencing of thousands of organisms completed or in progress, there is a growing need to integrate gene prediction with metabolic network analysis. Using Chlamydomonas reinhardtii as a model, we describe a systems-level methodology bridging metabolic network reconstruction with experimental verification of enzyme encoding open reading frames. Our quantitative and predictive metabolic model and its associated cloned open reading frames provide useful resources for metabolic engineering.
|Alternate Journal||Nat. Methods|