Metabolic gene-deletion strains of Escherichia coli evolve to computationally predicted growth phenotypes.

TitleMetabolic gene-deletion strains of Escherichia coli evolve to computationally predicted growth phenotypes.
Publication TypeJournal Article
Year of Publication2004
AuthorsFong S, Palsson BØ
JournalNature genetics
Volume36
Issue10
Pagination1056-8
PubMed Date2004 Oct
ISSN1061-4036
KeywordsDirected Molecular Evolution, Escherichia coli, Gene Deletion, Genes, Bacterial, Models, Genetic, Phenotype
Abstract

Genome-scale metabolic models have a promising ability to describe cellular phenotypes accurately. Here we show that strains of Escherichia coli carrying a deletion of a single metabolic gene increase their growth rates (by 87% on average) during adaptive evolution and that the endpoint growth rates can be predicted computationally in 39 of 50 (78%) strains tested. These results show that computational models can be used to predict the eventual effects of genetic modifications.

Alternate JournalNat. Genet.
PubMed ID15448692

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