|Title||Metabolic gene-deletion strains of Escherichia coli evolve to computationally predicted growth phenotypes.|
|Publication Type||Journal Article|
|Year of Publication||2004|
|Authors||Fong S, Palsson BØ|
|PubMed Date||2004 Oct|
|Keywords||Directed Molecular Evolution, Escherichia coli, Gene Deletion, Genes, Bacterial, Models, Genetic, Phenotype|
Genome-scale metabolic models have a promising ability to describe cellular phenotypes accurately. Here we show that strains of Escherichia coli carrying a deletion of a single metabolic gene increase their growth rates (by 87% on average) during adaptive evolution and that the endpoint growth rates can be predicted computationally in 39 of 50 (78%) strains tested. These results show that computational models can be used to predict the eventual effects of genetic modifications.
|Alternate Journal||Nat. Genet.|