Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA.

TitleTranscriptional regulation of the fad regulon genes of Escherichia coli by ArcA.
Publication TypeJournal Article
Year of Publication2006
AuthorsCho B-K, Knight EM, Palsson BØ
JournalMicrobiology (Reading, England)
Volume152
IssuePt 8
Pagination2207-19
PubMed Date2006 Aug
ISSN1350-0872
KeywordsAnaerobiosis, Bacterial Outer Membrane Proteins, Bacterial Proteins, Base Sequence, Escherichia coli, Escherichia coli Proteins, Fatty Acids, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Oxidation-Reduction, Regulon, Repressor Proteins, Transcription, Genetic
Abstract

ArcA is a global transcription factor required for optimal growth of Escherichia coli during anaerobic growth. In this study, the role of ArcA on the transcriptional regulatory subnetwork of the fad regulon was investigated. Gene expression profiles of deletion mutants (Delta arcA, Delta fadR and Delta arcA/Delta fadR) indicated that (i) ArcA is a major transcription factor for the transcriptional regulation of fatty acid metabolism in the absence of oxygen, and (ii) ArcA and FadR cooperatively regulate the fad regulon under anaerobic conditions. To determine the direct interaction between ArcA and the promoters of the fad regulon genes, chromatin immunoprecipitation (ChIP) analysis was performed. ChIP analysis suggested that ArcA directly binds to the promoter regions of the fad regulon genes in vivo. An ArcA-binding motif was identified from known binding sequences and predicted putative binding sites in the promoter regions of the fad regulon genes. These results indicate that ArcA directly represses the expression of fad regulon genes during anaerobic growth.

Alternate JournalMicrobiology (Reading, Engl.)
PubMed ID16849788

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