Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale.

TitleComparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale.
Publication TypeJournal Article
Year of Publication2006
AuthorsHerring CD, Raghunathan A, Honisch C, Patel T, Applebee KM, Joyce AR, Albert TJ, Blattner FR, van den Boom D, Cantor CR, Palsson BØ
JournalNature genetics
Volume38
Issue12
Pagination1406-12
PubMed Date2006 Dec
ISSN1061-4036
KeywordsAdaptation, Physiological, Culture Media, Directed Molecular Evolution, Escherichia coli, Genome, Bacterial, Genotype, Glycerol, Mutagenesis, Site-Directed, Mutation, Selection, Genetic, Time Factors
Abstract

We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We identified 13 different de novo mutations in five different E. coli strains and monitored their fixation over a 44-d period of adaptation. We obtained proof that the observed spontaneous mutations were responsible for improved fitness by creating single, double and triple site-directed mutants that had growth rates matching those of the evolved strains. The success of this new genome-scale approach indicates that real-time evolution studies will now be practical in a wide variety of contexts.

Alternate JournalNat. Genet.
PubMed ID17086184

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