|Title||Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Herring CD, Raghunathan A, Honisch C, Patel T, Applebee KM, Joyce AR, Albert TJ, Blattner FR, van den Boom D, Cantor CR, Palsson BØ|
|PubMed Date||2006 Dec|
|Keywords||Adaptation, Physiological, Culture Media, Directed Molecular Evolution, Escherichia coli, Genome, Bacterial, Genotype, Glycerol, Mutagenesis, Site-Directed, Mutation, Selection, Genetic, Time Factors|
We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We identified 13 different de novo mutations in five different E. coli strains and monitored their fixation over a 44-d period of adaptation. We obtained proof that the observed spontaneous mutations were responsible for improved fitness by creating single, double and triple site-directed mutants that had growth rates matching those of the evolved strains. The success of this new genome-scale approach indicates that real-time evolution studies will now be practical in a wide variety of contexts.
|Alternate Journal||Nat. Genet.|