Genome-scale metabolic reconstructions of multiple Salmonella strains reveal serovar-specific metabolic traits.

TitleGenome-scale metabolic reconstructions of multiple Salmonella strains reveal serovar-specific metabolic traits.
Publication TypeJournal Article
Year of Publication2018
AuthorsSeif Y, Kavvas E, Lachance J-C, Yurkovich JT, Nuccio S-P, Fang X, Catoiu E, Raffatellu M, Palsson BO, Monk JM
JournalNat Commun
Volume9
Issue1
Pagination3771
PubMed Date09/2018
ISSN2041-1723
Abstract

Salmonella strains are traditionally classified into serovars based on their surface antigens. While increasing availability of whole-genome sequences has allowed for more detailed subtyping of strains, links between genotype, serovar, and host remain elusive. Here we reconstruct genome-scale metabolic models for 410 Salmonella strains spanning 64 serovars. Model-predicted growth capabilities in over 530 different environments demonstrate that: (1) the Salmonella accessory metabolic network includes alternative carbon metabolism, and cell wall biosynthesis; (2) metabolic capabilities correspond to each strain's serovar and isolation host; (3) growth predictions agree with 83.1% of experimental outcomes for 12 strains (690 out of 858); (4) 27 strains are auxotrophic for at least one compound, including L-tryptophan, niacin, L-histidine, L-cysteine, and p-aminobenzoate; and (5) the catabolic pathways that are important for fitness in the gastrointestinal environment are lost amongst extraintestinal serovars. Our results reveal growth differences that may reflect adaptation to particular colonization sites.

Alternate JournalNat Commun
PubMed ID30218022
PubMed Central IDPMC6138749
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